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On April 26, 2020, Zhu Ying, a research team from the Institute of disease and students, Zhejiang Academy of Agricultural Sciences, was in plant cell & amp; Environment published a research paper entitled "high temperature in hibits the acquisition of storage materials by inducing alternative splicing of osbzip58 during filling stage in rice" online. The paper revealed that the variable shearing of osbzip58 was closely related to the high temperature tolerance of rice during filling stage.
High temperature can inhibit the accumulation of storage material, which has a negative effect on rice grain filling. However, the regulatory mechanism of this inhibition is still unclear.
In this study, it was reported that osbzip58, a transcription factor of opaque2 like, is a key factor regulating the accumulation of storage substances under high temperature. Under high temperature, osbzip58 gene promoted the expression of many seed storage protein genes and starch synthesis genes, but inhibited the expression of some starch hydrolytic α - amylase genes. Under high temperature, the loss of osbzip58 function will cause the endosperm to become powdery and atrophic, and the storage substance of seeds will decrease sharply. It was found that high temperature affected the selective splicing of osbzip58 and promoted the formation of truncated osbzip58 β protein form, rather than the formation of full-length osbzip58 α protein form. The transcriptional activity of osbzip58 β is lower than that of osbzip58 α, especially at high temperature. Interestingly, rice varieties with low heat sensitivity reduced the alternative splicing of osbzip58.
In conclusion, osbzip58 gene is the key gene to regulate the accumulation of storage materials under high temperature stress, and the decrease of alternative splicing of osbzip58 may be beneficial to the heat tolerance of rice during the filling process.
Effect of osbzip58 mutation on seed characters at room and high temperature
Attachment:
Abstract
High temperature (HT) has an adverse effect on rice grain filling by inhibiting the accumulation of storage materials. However, the regulatory mechanism of this inhibition remains unknown. Here, we report that Opaque2 like transcription factor OsbZIP58 is a key factor regulating storage material accumulation under HT. The OsbZIP58 gene promotes expression of many seed storage protein genes and starch synthesis genes while inhibits expression of some starch hydrolyzing α-amylase genes under HT. The loss of OsbZIP58 function leads to floury and shrunken endosperms and dramatically reduced storage materials in the seeds under HT. HT is found to affect alternative splicing of OsbZIP58, promoting the formation of the truncated OsbZIP58β protein form over the full-length OsbZIP58α protein form. The OsbZIP58β form has a lower transcriptional activity than the OsbZIP58α form, especially under HT condition. Interestingly, rice varieties with less heat sensitivity have reduced alternative splicing of OsbZIP58. Therefore, OsbZIP58 is a crucial gene in regulating storage material accumulation under HT and lower alternative splicing of OsbZIP58 may contribute to heat tolerance during grain filling.
Article link: https://onlinelibrary.wiley.com/doi/10.1111/pce.13779
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