Recently, Professor Zhang Liying, Department of pharmaceutical analysis, School of pharmacy, Nanjing Medical University, published a research paper entitled "a cell phone based acoustic platform for quantitative point of care testing" in ACS Nano (if: 13.903), a famous journal in the field of nano research, as the first author.
In this paper, the acoustic flow control technology and fluorescence visualization detection are combined to build a point of care testing (POCT) platform that can be used for biomarkers. The research results were completed by Professor Zhang Liying and Professor Tony Jun Huang (corresponding author) of Duke University.
Acoustic flow control is a new interdisciplinary science involving acoustics, chemistry, fluid physics, microelectronics and new materials, that is, the combination of acoustics and microfluidic technology, using sound wave as the driving force to realize the manipulation of fluid or particles in the microchannel. In this study, when the sound wave is turned off, the fluorescent nanoparticles are randomly scattered in the microchannel, and the fluorescence is weak. When the sound wave is applied to the microfluidic channel, the nanoparticles are continuously gathered under the action of the sound wave. Through the imaging function of the mobile phone, strong fluorescence can be observed. In order to achieve the visual detection of the target protein (heme), the author introduced red fluorescent nanoparticles and green fluorescent nanoparticles (internal standard) as ratio probes in the detection system. With the addition of the target, the red fluorescence gradually weakens, while the green fluorescence remains unchanged. On the screen of the mobile phone, there is a change from orange to yellow to green in the wide chromaticity of the fluorescence, and the change is related to the concentration of the target, realizing the visual semi quantitative detection.
The detection platform constructed by the research institute has the advantages of low cost, small sample dosage (10 μ L), fast detection speed (2 min) and convenient reading of results. In addition, by changing the type of fluorescent probe, it can realize the visualization and rapid detection of different biomarkers, which provides the possibility for the development of clinical diagnosis and bedside detection.
The research was supported by Jiangsu Provincial Government Scholarship for studying abroad, and was completed by Zhang Liying during her visit to Duke University.
Paper link: https://doi.org/10.1021/acsnano.9b08349
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