Article: Huake licorice and Yuan Wenxia can post the message on April 17. According to foreign media stat, ridcivir of Gilead has shown positive early results in a new clinical trial. However, due to the lack of clinical cases, it was finally "stopped". The previous treatment of new coronavirus with lopinavir also failed. Recently, researchers from Shanghai Institute of medicine, University of Chinese Academy of Sciences, China Pharmaceutical University, Shanghai Advanced Research Institute of Chinese Academy of Sciences, Wuhan virus Institute, Shanghai University of science and technology and other units have made joint efforts to start with traditional drugs and found that baicalin and Baicalin in traditional Chinese medicine baicalin have obvious inhibitory effect on new coronavirus 。 Baicalin and baicalein can inhibit the key protease of new coronavirus
In order to evaluate the antiviral activity and biosafety of baicalin and Baicalin in detail, the researchers selected Vero E6, an African green monkey kidney cell line infected with sars-cov-2, and Vero E6, a normal African green monkey kidney cell line, as the experimental subjects. The antiviral activity of baicalin and baicalein against sars-cov-2 in Vero E6 cells. The cytotoxicity of baicalin (a) or baicalin (b) on Vero E6 cells was determined by CCK-8. Under different concentrations of baicalin (c) or baicalein (d), sars-cov-2 infected cells with a complex number of 0.05 (MOI) for 48 hours. Then the virus content in the supernatant was quantified by QRT PCR. In the experiment, baicalin and baicalein can inhibit the new coronavirus in a small dose. The maximum half effective concentrations of the two compounds were 10.27 and 1.69 μ m, respectively. The experimental results show that the antiviral activity of the two compounds is better than that of other compounds. And the cytotoxic concentration (cc50) of these two compounds is more than 200 μ m, which shows that the side effects of the two components of traditional Chinese medicine are very low. Scutellaria baicalensis can inhibit the replication of 3clpro, an important protease in the new coronavirus. Baicalin and baicalein were identified as the first non covalent and non polypeptide inhibitors of sars-cov-2clpro. The unique binding mode of scutellarin and sars-cov-3 CLPRO is noteworthy that the binding mode of scutellarin and sars-cov-2 3clpro determined by X-ray protein crystallography is significantly different from that of known inhibitors. Baicalein perfectly binds to the center of the substrate binding site. It acts as a "shield" in front of the catalytic dimer by interacting with two catalytic residues (the key S1 / S2 sub site and the oxygen anion ring) to prevent the peptide substrate from approaching the active site. Baicalein can bind to leu141 / gly141 / ser144 through a series of direct and indirect hydrogen bonds, thus fixing the free oxygen anion ring structure to stabilize the tetrahedral transition state in protein hydrolysis. A series of results showed that scutellarin can recognize the molecular details of sars-cov-23 CLPRO, and explained the possible reason why scutellarin can significantly inhibit the activity of sars-cov-23 CLPRO. Baicalin and baicalein -- baicalin and baicalin, the gifts of nature, are flavonoids extracted from the dry roots of Scutellaria baicalensis Georgi. It has significant biological activity, with bacteriostatic, diuretic, anti-inflammatory, cholesterol lowering, antithrombotic, asthmatic, purgative fire and detoxification, hemostasis, foetus, antiallergic and antispasmodic effects. It is also a specific inhibitor of sialidase in mammalian liver, with the function of regulating some diseases, and also has a strong physiological effect of anti-cancer reaction. Baicalein mainly exists in Scutellaria baicalensis Georgi, which is one of the highest content of flavonoids in Scutellaria baicalensis Georgi. Baicalein has anti-inflammatory, anti allergic, diuretic, cholagogic, cholesterol lowering, antithrombotic, asthmatic and other effects. It is also a specific inhibitor of sialidase in mammalian liver, which can regulate some diseases. In industry, baicalin phosphate is made from baicalin phosphate, which is used in allergic and asthmatic pneumonia, as astringent and hemostatic. Traditional Chinese medicine is the treasure of Chinese medicine. New coronavirus is sweeping the world. How to deal with this sudden pandemic? Xiaobian thinks it is an important strategy to find the answer from traditional Chinese medicine. It is believed that after baicalin and baicalein, the components of Scutellaria baicalensis Georgi, have been proved to have significant anti coronavirus activity, more related researches will be carried out in succession, and baicalin and baicalein will be expected to enter the clinical practice and become the sword of hope for anti coronavirus. Reference: https://www.biorxiv.org/content/10.1101/2020.04.13.038687v1
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